Calcium binding by human erythrocyte membranes. Significance of carboxyl, amino and thiol groups
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چکیده
منابع مشابه
Thiol methyltransferase activity in colonocytes and erythrocyte membranes.
AIMS To verify the improved thiol methyltransferase (TMT) assay and measure activity in isolated colonocytes and erythrocyte membranes of the same subjects. METHODS High performance liquid chromatography with radioactivity detection was used to measure 14C-methylmercaptoethanol formation, the reaction product of cell extracts incubated with mercaptoethanol and 14C-S-adenosylmethionine. RESU...
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The cell membrane calcium binding capacity of genetically hypertensive rats is reduced when measured in the presence of the submicromolar calcium concentrations proper of intracellular environment. The present work, performed as an ancillary study to an epidemiological survey on an entire population, aimed to investigate the existence of a similar abnormality in human hypertension. Calcium bind...
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Calcium and magnesium contents of whole erythrocytes and of erythrocyte membranes (stroma) were determined by atomic absorption technique on five mammalian species, man, rabbit, pig, cow and dog. In every species, the magnesium content of the whole erythrocyte is predominantly higher than the calcium content. Of the total magnesium, about 2-6% are localized in the membrane, whereas 40-86% of th...
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Understanding the membrane solubilization process and finding effective solubilizing agents are crucial challenges in biochemical research. Here we report results on the interaction of the novel linear alkylamido propyl dimethyl amino propanosulfonate detergents, ASB-14 and ASB-16, with human erythrocyte membranes. An estimation of the critical micelle concentration of these zwitterionic deterg...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1971
ISSN: 0306-3283
DOI: 10.1042/bj1250343